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In all , we obtained 22,270 transgenic RNAi lines , representing 13,251 RNAi constructs and 12,088 genes ( 88.0 % of predicted protein - coding genes ; Supplementary Table 2 ) . by fewer 19 - mers , but at least one , is considered a ...
In all , we obtained 22,270 transgenic RNAi lines , representing 13,251 RNAi constructs and 12,088 genes ( 88.0 % of predicted protein - coding genes ; Supplementary Table 2 ) . by fewer 19 - mers , but at least one , is considered a ...
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This is consistent with the partial reduction in target mRNA levels generally observed for these lines ( Fig . 2a ) . We wondered whether overexpression of components of the RNAi machinery might enhance such phenotypes .
This is consistent with the partial reduction in target mRNA levels generally observed for these lines ( Fig . 2a ) . We wondered whether overexpression of components of the RNAi machinery might enhance such phenotypes .
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Transgenic RNAi lines . Germline transformation was performed using a hyperactive P - element transposase “ 0 . For verification we extracted genomic DNA from single flies in 96 - well plates and performed PCR with one gene - specific ...
Transgenic RNAi lines . Germline transformation was performed using a hyperactive P - element transposase “ 0 . For verification we extracted genomic DNA from single flies in 96 - well plates and performed PCR with one gene - specific ...
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Bibliografski podatki
| Naslov | Nature, Količina 448 |
| Urednik | Sir Norman Lockyer |
| Založnik | Macmillan Journals Limited, 2007 |
| Vir izvirnika | Univerza Illinois (Urbana in Champaign) |
| Pretvorjeno v digitalni zapis | 12 dec 2018 |
| Izvozi navedbo | BiBTeX EndNote RefMan |